Abstract
Using a human CD25 reporter transgene controlled by regulatory sequences from the gene encoding pre-T cell receptor alpha, we identified a common lymphocyte precursor (CLP-2) population that, in contrast to the previously identified CLP-1 population, was c-Kit-B220+. In short-term culture, the CLP-2 could be derived from the CLP-1 subset, and contained cells that in clonogenic assays were assessed to be bipotent precursors of T and B cells. Intravenous injection of bone marrow cells yielded a selective accumulation of CLP-2 thymic immigrants that in thymic organ culture generated mature alphabeta T cells. Although the CLP-2 subset may represent the most differentiated population with T cell potential before commitment to the B cell lineage, other subsets of thymic immigrants capable of generating T cells may exist.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antigens, CD19 / genetics
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Antigens, CD19 / immunology
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B-Lymphocytes / cytology
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B-Lymphocytes / immunology*
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Bone Marrow Cells / cytology
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Bone Marrow Cells / immunology*
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Cell Differentiation / immunology
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Cell Lineage
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Cell Movement / immunology
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Flow Cytometry
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Gene Expression Regulation
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Leukocyte Common Antigens / genetics
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Leukocyte Common Antigens / immunology*
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Mice, Transgenic
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Proto-Oncogene Proteins c-kit / genetics
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Proto-Oncogene Proteins c-kit / immunology
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RNA / chemistry
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RNA / genetics
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Receptors, Interleukin-2 / genetics
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Receptors, Interleukin-2 / immunology
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Reverse Transcriptase Polymerase Chain Reaction
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T-Lymphocytes / cytology
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T-Lymphocytes / immunology*
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Thymus Gland / cytology
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Thymus Gland / immunology*
Substances
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Antigens, CD19
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Receptors, Interleukin-2
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RNA
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Proto-Oncogene Proteins c-kit
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Leukocyte Common Antigens