Sensitivity of genera Porphyromonas and Prevotella to the bactericidal action of C-terminal domain of human CAP18 and its analogues

Oral Microbiol Immunol. 2003 Oct;18(5):329-32. doi: 10.1034/j.1399-302x.2003.00083.x.

Abstract

This paper reports the effect of the synthesized 27-amino acid sequence in the C-terminal domain of human CAP18 (hCAP18), a human cationic antibacterial protein or cathelicidin, on certain strains belonging to the genera Porophyromonas and Prevotella. The domain binds lipopolysaccharides (LPS) from Porophyromonas gingivalis and Porophyromonas circumdentaria as well as enterobacterial LPS. Two analogues of hCAP18, designated LL/CAP18 and FF/CAP18, were also tested to determine whether additional activity was obtained. The analogue peptides replaced with hydrophobic and cationic amino acid residues showed more potent bactericidal and LPS-binding activities than the original one.

MeSH terms

  • Antimicrobial Cationic Peptides / pharmacology*
  • Cathelicidins
  • Humans
  • Lipopolysaccharides / antagonists & inhibitors*
  • Multigene Family / drug effects*
  • Porphyromonas / classification
  • Porphyromonas / drug effects*
  • Porphyromonas gingivalis / drug effects
  • Prevotella / drug effects*
  • Prevotella intermedia / drug effects
  • Prevotella melaninogenica / drug effects

Substances

  • Antimicrobial Cationic Peptides
  • Cathelicidins
  • Lipopolysaccharides