First enzymatic synthesis of an N1-cyclised cADPR (cyclic-ADP ribose) analogue with a hypoxanthine partial structure: discovery of a membrane permeant cADPR agonist

Gerd K Wagner; Steven Black; Andreas H Guse; Barry V L Potter

doi:10.1039/b305660k

First enzymatic synthesis of an N1-cyclised cADPR (cyclic-ADP ribose) analogue with a hypoxanthine partial structure: discovery of a membrane permeant cADPR agonist

Chem Commun (Camb). 2003 Aug 7:(15):1944-5. doi: 10.1039/b305660k.

Authors

Gerd K Wagner¹, Steven Black, Andreas H Guse, Barry V L Potter

Affiliation

¹ University of Bath, Department of Pharmacy and Pharmacology, Wolfson Laboratory of Medicinal Chemistry, Claverton Down, Bath, UK BA2 7AY.

PMID: 12932045
DOI: 10.1039/b305660k

Abstract

Nicotinamide 8-Br-hypoxanthine dinucleotide (8-Br-NHD+) was cyclised at the N1 position by the ADP-ribosyl cyclase from Aplysia californica to give cyclic 8-Br-inosine diphosphoribose (8-Br-N1-cIDPR), a novel membrane-permeant agonist of Ca2+ release in human T cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

ADP-ribosyl Cyclase / metabolism
Cell Membrane / drug effects
Cell Membrane / metabolism*
Copper / pharmacology
Cyclic ADP-Ribose / analogs & derivatives*
Cyclic ADP-Ribose / biosynthesis*
Cyclic ADP-Ribose / chemistry
Humans
Hypoxanthine / metabolism*
Jurkat Cells / drug effects
Jurkat Cells / metabolism

Substances

Cyclic ADP-Ribose
Hypoxanthine
Copper
ADP-ribosyl Cyclase