Abstract
A new plasmid-mediated TEM-derived extended-spectrum beta-lactamase, TEM-91, was identified in a ceftazidime-resistant (MIC, >128 microg per ml) Escherichia coli strain isolated in 1996 in Japan. TEM-91 has three amino acid substitutions, R164C, M184T, and E240K, compared with TEM-1 penicillinase. The isoelectric point (pI), K(m), and k(cat) of TEM-91 for ceftazidime were 5.7, 179 microM, and 29.0 s(-1), respectively. The K(i) of clavulanic acid for ceftazidime hydrolysis was 30.3 nM.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Substitution
-
Anti-Bacterial Agents / metabolism
-
Anti-Bacterial Agents / pharmacology
-
Ceftazidime / pharmacology*
-
Cephalosporin Resistance / genetics*
-
Cephalosporins / pharmacology*
-
Chromatography, High Pressure Liquid
-
DNA, Bacterial / analysis
-
DNA, Bacterial / genetics
-
Drug Combinations
-
Enzyme Inhibitors / pharmacology
-
Escherichia coli / enzymology
-
Escherichia coli / genetics
-
Escherichia coli Infections / microbiology
-
Escherichia coli Infections / urine
-
Escherichia coli Proteins / antagonists & inhibitors
-
Escherichia coli Proteins / genetics*
-
Escherichia coli Proteins / metabolism*
-
Humans
-
Kinetics
-
Molecular Sequence Data
-
Point Mutation / genetics
-
beta-Lactamase Inhibitors
-
beta-Lactamases / genetics*
-
beta-Lactamases / metabolism*
Substances
-
Anti-Bacterial Agents
-
Cephalosporins
-
DNA, Bacterial
-
Drug Combinations
-
Enzyme Inhibitors
-
Escherichia coli Proteins
-
beta-Lactamase Inhibitors
-
Ceftazidime
-
beta-Lactamases
-
TEM-91 beta-lactamase, E coli