Abstract
Differing spatial scales of signaling cascades are critical for cell orientation during chemotactic responses. We used biotin EGF bound to streptavidin-coupled magnetic beads to locally stimulate cells overexpressing the EGF receptor. We have found that EGF-induced actin polymerization remains localized even under conditions of receptor overexpression. Conversely, EGF-induced ERK activation spreads throughout the cell body after EGF bead stimulation. The localized actin polymerization is independent of PI3-kinase and rho protein activity and requires Arp2/3 complex and cofilin function. Thus, we find differing spatial scales of signaling from the EGF receptor, supporting models of chemotaxis that integrate short- and long-range signaling.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Actin Depolymerizing Factors
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Actins / metabolism
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Androstadienes / metabolism
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Animals
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Cell Line, Tumor
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Enzyme Inhibitors / metabolism
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Epidermal Growth Factor / metabolism
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ErbB Receptors / metabolism*
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Humans
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Microfilament Proteins / genetics
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Microfilament Proteins / metabolism
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Mitogen-Activated Protein Kinases / metabolism
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Phosphatidylinositol 3-Kinases / metabolism
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Phosphotyrosine / metabolism
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Protein Serine-Threonine Kinases*
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Proto-Oncogene Proteins / metabolism
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Proto-Oncogene Proteins c-akt
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RNA, Small Interfering / metabolism
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Rats
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Signal Transduction / physiology*
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Wortmannin
Substances
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Actin Depolymerizing Factors
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Actins
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Androstadienes
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Enzyme Inhibitors
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Microfilament Proteins
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Proto-Oncogene Proteins
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RNA, Small Interfering
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Phosphotyrosine
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Epidermal Growth Factor
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ErbB Receptors
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Protein Serine-Threonine Kinases
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Proto-Oncogene Proteins c-akt
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Mitogen-Activated Protein Kinases
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Wortmannin