Upregulation of uncoupling proteins by oral administration of capsiate, a nonpungent capsaicin analog

Yoriko Masuda; Satoshi Haramizu; Kasumi Oki; Koichiro Ohnuki; Tatsuo Watanabe; Susumu Yazawa; Teruo Kawada; Shu-ichi Hashizume; Tohru Fushiki

doi:10.1152/japplphysiol.00828.2002

Upregulation of uncoupling proteins by oral administration of capsiate, a nonpungent capsaicin analog

J Appl Physiol (1985). 2003 Dec;95(6):2408-15. doi: 10.1152/japplphysiol.00828.2002. Epub 2003 Sep 5.

Authors

Yoriko Masuda¹, Satoshi Haramizu, Kasumi Oki, Koichiro Ohnuki, Tatsuo Watanabe, Susumu Yazawa, Teruo Kawada, Shu-ichi Hashizume, Tohru Fushiki

Affiliation

¹ Laboratory of Nutrition Chemistry, Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto Univ., Kyoto 606-8502, Japan.

PMID: 12959953
DOI: 10.1152/japplphysiol.00828.2002

Abstract

Capsiate is a nonpungent capsaicin analog, a recently identified principle of the nonpungent red pepper cultivar CH-19 Sweet. In the present study, we report that 2-wk treatment of capsiate increased metabolic rate and promoted fat oxidation at rest, suggesting that capsiate may prevent obesity. To explain these effects, at least in part, we examined uncoupling proteins (UCPs) and thyroid hormones. UCPs and thyroid hormones play important roles in energy expenditure, the maintenance of body weight, and thermoregulation. Two-week treatment of capsiate increased the levels of UCP1 protein and mRNA in brown adipose tissue and UCP2 mRNA in white adipose tissue. This dose of capsiate did not change serum triiodothyronine or thyroxine levels. A single dose of capsiate temporarily raised both UCP1 mRNA in brown adipose tissue and UCP3 mRNA in skeletal muscle. These results suggest that UCP1 and UCP2 may contribute to the promotion of energy metabolism by capsiate, but that thyroid hormones do not.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adipose Tissue / drug effects
Adipose Tissue, Brown / metabolism
Animals
Blotting, Western
Capsaicin / analogs & derivatives*
Capsaicin / pharmacology*
Carbohydrate Metabolism
Carrier Proteins / biosynthesis
Carrier Proteins / metabolism*
Epididymis / metabolism
Gases / metabolism
Ion Channels
Male
Membrane Proteins / metabolism*
Membrane Transport Proteins / biosynthesis
Mice
Mitochondrial Proteins / biosynthesis
Muscle, Skeletal / metabolism
Organ Size / drug effects
Oxygen Consumption / drug effects
RNA / biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
Thyroid Hormones / blood
Uncoupling Protein 1
Uncoupling Protein 2
Uncoupling Protein 3
Up-Regulation / drug effects*

Substances

Carrier Proteins
Gases
Ion Channels
Membrane Proteins
Membrane Transport Proteins
Mitochondrial Proteins
Thyroid Hormones
Ucp1 protein, mouse
Ucp2 protein, mouse
Ucp3 protein, mouse
Uncoupling Protein 1
Uncoupling Protein 2
Uncoupling Protein 3
RNA
capsiate
Capsaicin