Array painting: a method for the rapid analysis of aberrant chromosomes using DNA microarrays

J Med Genet. 2003 Sep;40(9):664-70. doi: 10.1136/jmg.40.9.664.

Abstract

Objective: The authors describe a method, termed array painting, which allows the rapid, high resolution analysis of the content and breakpoints of aberrant chromosomes.

Methods: Array painting is similar in concept to reverse chromosome painting and involves the hybridisation of probes generated by PCR of small numbers of flow sorted chromosomes on large insert genomic clone DNA microarrays.

Results: and

Conclusions: By analysing patients with cytogenetically balanced chromosome rearrangements, the authors show the effectiveness of array painting as a method to map breakpoints prior to cloning and sequencing chromosome rearrangements.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Cell Line
  • Child
  • Child, Preschool
  • Chromosome Aberrations*
  • Chromosome Disorders / genetics
  • Chromosome Disorders / pathology
  • Chromosomes, Human, Pair 11 / genetics
  • Chromosomes, Human, Pair 12 / genetics
  • Chromosomes, Human, Pair 17 / genetics
  • Chromosomes, Human, Pair 22 / genetics
  • Female
  • Flow Cytometry
  • Humans
  • In Situ Hybridization, Fluorescence / methods
  • Karyotyping / methods
  • Male
  • Oligonucleotide Array Sequence Analysis / methods*
  • Translocation, Genetic