Expression of alpha2a-2b neuregulin-1 is associated with early peripheral nerve repair along muscle-enriched tubes

Neuroreport. 2003 Aug 6;14(11):1541-5. doi: 10.1097/00001756-200308060-00029.

Abstract

Using RT-PCR, we have investigated expression of isoforms beta1 (the axonal isoform) and alpha2a-2b (the mesenchymal isoform) of neuregulin-1, one of the most important known trophic factors for Schwann cells, in the rat sciatic nerve repaired by muscle-enriched non-nervous conduits (made by a vein filled with fresh skeletal muscle). Repaired nerves were harvested 2, 6 and 13 days post-operatively. Results showed that while muscle-vein combined grafts were enriched in mRNA coding for alpha2a-2b since the very early regeneration stages, isoform beta1 mRNA was not detectable inside the tubes at day 2 and 6 post-operatively while its expression at day 13 was very slight. These results suggest that Schwann cell survival and activity inside a fresh muscle-enriched non-nervous conduit graft (a key factor for successful nerve regeneration along the graft) may be supported by the mesenchymal isoform of neuregulin-1 during very early repair phases, i.e. when axons are still not present along the tube.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Survival
  • DNA Primers
  • DNA, Complementary / biosynthesis
  • DNA, Complementary / genetics
  • Densitometry
  • Kinetics
  • Male
  • Muscle, Skeletal / innervation*
  • Muscle, Skeletal / physiology
  • Muscle, Smooth, Vascular / cytology
  • Muscle, Smooth, Vascular / drug effects
  • Muscle, Smooth, Vascular / innervation
  • Nerve Regeneration / physiology*
  • Neuregulin-1 / biosynthesis*
  • Neuregulin-1 / genetics*
  • Peripheral Nerves / physiology*
  • Rats
  • Reverse Transcriptase Polymerase Chain Reaction
  • Schwann Cells / physiology
  • Transplantation, Autologous

Substances

  • DNA Primers
  • DNA, Complementary
  • Neuregulin-1