We monitored the total and Perennial lolium-specific IgE, IgG, IgM and IgA serum levels of pollinotic patients under three different immunotherapeutic treatments with gramineal pollens over a one-year period. We found statistically significant (p < 0.05) decreases in the total serum IgE levels in successive controls performed after 3, 6 and 12 month's treatment with respect to the basal control levels. The specific IgE levels also decreased gradually throughout the study, while the total serum IgG and IgM levels increased significantly over the first 3 months, and those of IgG continued to increase up to the sixth month. Finally, the total serum IgA levels did not change significantly during the first year of treatment of our pollinotic patients.