Abstract
We investigated the effects of treatment with anti-parathyroid hormone-related protein (1-34) monoclonal murine antibody (anti-PTHrP MoAb) on apoptosis and the differentiation of chondrosarcoma HTB-94 cells. Treatment with anti-PTHrP MoAb accelerated apoptosis of HTB-94 cells in a dose-dependent manner, and anti-PTHrP MoAb also promoted the chondrogenic differentiation of HTB-94 cells. The induction of apoptosis by anti-PTHrP MoAb via imbalance of Bcl-2/Bax ratio and activation of caspase-3 may provide a mechanistic explanation for its potential antitumor effects. Our results suggest the possibility that anti-PTHrP MoAb may be beneficial as a new treatment for chondrosarcoma.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Antibodies, Monoclonal / pharmacology*
-
Apoptosis / drug effects*
-
Bone Neoplasms / immunology
-
Bone Neoplasms / metabolism
-
Bone Neoplasms / pathology*
-
Caspase 3
-
Caspases / metabolism
-
Cell Differentiation / drug effects*
-
Cell Division / drug effects
-
Chondrosarcoma / immunology
-
Chondrosarcoma / metabolism
-
Chondrosarcoma / pathology*
-
Collagen Type II / genetics
-
Collagen Type II / metabolism
-
Collagen Type X / genetics
-
Collagen Type X / metabolism
-
DNA Primers / chemistry
-
Humans
-
Immunoenzyme Techniques
-
Parathyroid Hormone-Related Protein / immunology*
-
Peptide Fragments / immunology
-
Proto-Oncogene Proteins / genetics
-
Proto-Oncogene Proteins / metabolism
-
Proto-Oncogene Proteins c-bcl-2 / genetics
-
Proto-Oncogene Proteins c-bcl-2 / metabolism
-
Reverse Transcriptase Polymerase Chain Reaction
-
Tumor Cells, Cultured
-
bcl-2-Associated X Protein
Substances
-
Antibodies, Monoclonal
-
BAX protein, human
-
Collagen Type II
-
Collagen Type X
-
DNA Primers
-
Parathyroid Hormone-Related Protein
-
Peptide Fragments
-
Proto-Oncogene Proteins
-
Proto-Oncogene Proteins c-bcl-2
-
bcl-2-Associated X Protein
-
CASP3 protein, human
-
Caspase 3
-
Caspases