Monoclonal antibody to parathyroid hormone-related protein induces differentiation and apoptosis of chondrosarcoma cells

Cancer Lett. 2003 Sep 25;199(2):147-55. doi: 10.1016/s0304-3835(03)00347-1.

Abstract

We investigated the effects of treatment with anti-parathyroid hormone-related protein (1-34) monoclonal murine antibody (anti-PTHrP MoAb) on apoptosis and the differentiation of chondrosarcoma HTB-94 cells. Treatment with anti-PTHrP MoAb accelerated apoptosis of HTB-94 cells in a dose-dependent manner, and anti-PTHrP MoAb also promoted the chondrogenic differentiation of HTB-94 cells. The induction of apoptosis by anti-PTHrP MoAb via imbalance of Bcl-2/Bax ratio and activation of caspase-3 may provide a mechanistic explanation for its potential antitumor effects. Our results suggest the possibility that anti-PTHrP MoAb may be beneficial as a new treatment for chondrosarcoma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / pharmacology*
  • Apoptosis / drug effects*
  • Bone Neoplasms / immunology
  • Bone Neoplasms / metabolism
  • Bone Neoplasms / pathology*
  • Caspase 3
  • Caspases / metabolism
  • Cell Differentiation / drug effects*
  • Cell Division / drug effects
  • Chondrosarcoma / immunology
  • Chondrosarcoma / metabolism
  • Chondrosarcoma / pathology*
  • Collagen Type II / genetics
  • Collagen Type II / metabolism
  • Collagen Type X / genetics
  • Collagen Type X / metabolism
  • DNA Primers / chemistry
  • Humans
  • Immunoenzyme Techniques
  • Parathyroid Hormone-Related Protein / immunology*
  • Peptide Fragments / immunology
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / metabolism
  • Proto-Oncogene Proteins c-bcl-2 / genetics
  • Proto-Oncogene Proteins c-bcl-2 / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tumor Cells, Cultured
  • bcl-2-Associated X Protein

Substances

  • Antibodies, Monoclonal
  • BAX protein, human
  • Collagen Type II
  • Collagen Type X
  • DNA Primers
  • Parathyroid Hormone-Related Protein
  • Peptide Fragments
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • bcl-2-Associated X Protein
  • CASP3 protein, human
  • Caspase 3
  • Caspases