Abstract
We investigated the frequency of BRAF mutations in human pancreatic cancer specimens to determine its role in the development of pancreatic cancer. Nine pancreatic cancer samples without a K-ras codon 12 mutation and 19 with a K-ras mutation were included in the study. Analyses of the BRAF sequence revealed mutations in exon 15 (V599E) in two cases, both of which also exhibited a K-ras codon 12 mutation. No BRAF mutation was found in cases without a K-ras mutation. The BRAF V599E mutation was not found to be a major mutation in pancreatic cancers that had no K-ras codon 12 mutation.
MeSH terms
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Adenocarcinoma / etiology
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Adenocarcinoma / genetics
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Adenocarcinoma, Mucinous / etiology
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Adenocarcinoma, Mucinous / genetics
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Aged
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Aged, 80 and over
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Carcinoma, Papillary / etiology
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Carcinoma, Papillary / genetics
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DNA Mutational Analysis
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DNA Primers / chemistry
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Female
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Genes, ras / genetics*
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Humans
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Male
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Middle Aged
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Mutation / genetics*
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Neoplasm Staging
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Oncogene Proteins / genetics*
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Pancreatic Neoplasms / etiology
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Pancreatic Neoplasms / genetics*
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Polymerase Chain Reaction
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Proto-Oncogene Proteins B-raf
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Survival Rate
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Tumor Cells, Cultured
Substances
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DNA Primers
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Oncogene Proteins
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BRAF protein, human
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Proto-Oncogene Proteins B-raf