Abstract
Human CCAAT displacement protein (CDP), a putative repressor of developmentally regulated gene expression, was purified from HeLa cells by DNA binding-site affinity chromatography. cDNA encoding CDP was obtained by immunoscreening a lambda gt11 library with antibody raised against purified protein. The deduced primary amino acid sequence of CDP reveals remarkable homology to Drosophila cut with respect to the presence of a unique homeodomain and "cut repeats". As cut participates in determination of cell fate in several tissues in Drosophila, the similarity predicts a broad role for CDP in mammalian development.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cloning, Molecular
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DNA / genetics
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Drosophila / genetics
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Drosophila Proteins
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Gene Expression
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HeLa Cells
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Homeodomain Proteins
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Humans
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Molecular Sequence Data
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Nerve Tissue Proteins / genetics*
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Nuclear Proteins / genetics*
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Repressor Proteins / genetics*
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Sequence Homology, Amino Acid
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Species Specificity
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Transcription Factors
Substances
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CUX1 protein, human
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Drosophila Proteins
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Homeodomain Proteins
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Nerve Tissue Proteins
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Nuclear Proteins
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Repressor Proteins
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Transcription Factors
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ct protein, Drosophila
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DNA