Abstract
To test models of T-cell recognition, mice transgenic for T-cell receptor alpha or beta chain have been immunized with variant peptides that force changes in the resulting T-cell response. In particular, charge substitutions on the peptide often elicit reciprocal charges in the junctional (CDR3) sequences of T-cell receptor V alpha or V beta chains, indicating direct T-cell receptor-peptide contact, and allowing derivation of a topology for the T-cell receptor-MHC interaction. At one position on the peptide, variants transformed a homogeneous V beta response into a very heterogeneous one.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Antigens, Differentiation, T-Lymphocyte / genetics
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Antigens, Differentiation, T-Lymphocyte / immunology
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Base Sequence
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Binding, Competitive
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CD3 Complex
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Columbidae
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Cytochrome c Group / immunology*
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Cytochrome c Group / metabolism
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Genetic Variation*
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Kinetics
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Lymph Nodes / immunology
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Lymphocyte Activation
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Lymphocytes / immunology
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Macromolecular Substances
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Major Histocompatibility Complex
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Mice
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Mice, Transgenic
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Models, Structural
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Molecular Sequence Data
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Moths
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Oligodeoxyribonucleotides
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Protein Conformation
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Receptors, Antigen, T-Cell / genetics
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Receptors, Antigen, T-Cell / immunology
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Receptors, Antigen, T-Cell / metabolism*
Substances
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Antigens, Differentiation, T-Lymphocyte
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CD3 Complex
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Cytochrome c Group
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Macromolecular Substances
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Oligodeoxyribonucleotides
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Receptors, Antigen, T-Cell