Abstract
The genus Phytomonas is responsible for many diseases in different crop plant species. The finding that chitin is an exposed cell surface polysaccharide in Phytomonas françai and the observation that chitinases can inhibit fungal growth raises expectations about the potential effect of plant chitinases on the P. françai cell membrane surface. The plant chitinases Urtica dioica agglutinin (UDA) and Arabidopsis thaliana Chia4 (ATCHIT4) proteins were over-expressed in bacteria and the interaction between these proteins and P. françai surface was analyzed by immunocytochemistry. We showed that UDA and ATCHIT4 proteins can interact with surface-exposed chitin from P. françai.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Arabidopsis / genetics*
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Arabidopsis Proteins / genetics*
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Arabidopsis Proteins / metabolism
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Carrier Proteins / genetics
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Carrier Proteins / metabolism
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Chitin / metabolism*
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Chitinases / genetics
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Chitinases / isolation & purification
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Chitinases / metabolism*
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Electrophoresis, Polyacrylamide Gel
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Flow Cytometry
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Immunologic Techniques
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Maltose-Binding Proteins
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Microscopy, Fluorescence
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Microscopy, Phase-Contrast
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Plant Lectins / genetics
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Plant Lectins / isolation & purification
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Plant Lectins / metabolism*
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / metabolism
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Trypanosomatina / chemistry
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Trypanosomatina / cytology
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Trypanosomatina / metabolism*
Substances
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Arabidopsis Proteins
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Carrier Proteins
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Maltose-Binding Proteins
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Plant Lectins
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Recombinant Fusion Proteins
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stinging nettle lectin
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Chitin
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Chitinases