K-sam gene encodes secreted as well as transmembrane receptor tyrosine kinase

Proc Natl Acad Sci U S A. 1992 Apr 1;89(7):2960-4. doi: 10.1073/pnas.89.7.2960.

Abstract

K-sam was first identified as a gene amplified in the stomach cancer cell line KATO-III. The size of the major transcript of the K-sam gene was 3.5 kilobases in KATO-III cells, and we have previously shown that K-sam encodes a receptor tyrosine kinase that belongs to the heparin-binding growth factor receptor, or fibroblast growth factor receptor, gene family. The K-sam gene expresses multiple sizes of mRNAs in brain tissue, the immature teratoma cell line NCC-IT, and KATO-III. RNA blot analyses with a variety of K-sam probes indicate that there are at least four classes of K-sam mRNAs. Three types of K-sam cDNAs in addition to the previously reported type of K-sam cDNA were isolated, and their nucleotide sequences encode a full-length transmembrane receptor, a secreted receptor with a tyrosine kinase domain, and a secreted receptor without a tyrosine kinase domain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA / genetics
  • Gene Expression
  • Humans
  • Molecular Sequence Data
  • Molecular Weight
  • Neoplasm Proteins / genetics*
  • Oligodeoxyribonucleotides / chemistry
  • Polymerase Chain Reaction
  • Protein-Tyrosine Kinases / genetics*
  • RNA, Messenger / genetics
  • Receptor Protein-Tyrosine Kinases*
  • Receptor, Fibroblast Growth Factor, Type 2
  • Receptors, Cell Surface / genetics*
  • Receptors, Fibroblast Growth Factor*

Substances

  • Neoplasm Proteins
  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, Fibroblast Growth Factor
  • DNA
  • FGFR2 protein, human
  • Protein-Tyrosine Kinases
  • Receptor Protein-Tyrosine Kinases
  • Receptor, Fibroblast Growth Factor, Type 2

Associated data

  • GENBANK/M87770
  • GENBANK/M87771
  • GENBANK/M87772