Abstract
The distribution of the messenger RNA coding for the recently cloned inositol 1,4,5-trisphosphate (InsP3) 3-kinase, the enzyme phosphorylating InsP3 to InsP4, was compared to the localizations of InsP3 receptor mRNA in the human brain using in situ hybridization histochemistry and oligonucleotide probes. InsP3 3-kinase and receptor mRNA levels were high in the cerebellar Purkinje cells. They were also observed, to a much lesser degree than in the cerebellum, in the hippocampal CA1 pyramidal cells and dentate gyrus granule cells, in the majority of the cortical neurons and in the striatal medium-sized neurons. Both mRNAs were not detected in the brainstem and in the glial cells.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Aged, 80 and over
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Brain Chemistry*
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Calcium Channels*
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Female
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Humans
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Inositol 1,4,5-Trisphosphate Receptors
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Male
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Nucleic Acid Hybridization
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Phosphotransferases (Alcohol Group Acceptor)*
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Phosphotransferases / analysis*
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Purkinje Cells / chemistry
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RNA, Messenger / analysis*
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Receptors, Cell Surface / analysis*
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Receptors, Cytoplasmic and Nuclear*
Substances
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Calcium Channels
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ITPR1 protein, human
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Inositol 1,4,5-Trisphosphate Receptors
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RNA, Messenger
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Receptors, Cell Surface
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Receptors, Cytoplasmic and Nuclear
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Phosphotransferases
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Phosphotransferases (Alcohol Group Acceptor)
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Inositol 1,4,5-trisphosphate 3-kinase