Oxidation of methylamine by a Paracoccus denitrificans mutant impaired in the synthesis of the bc1 complex and the aa3-type oxidase. Evidence for the existence of an alternative cytochrome c oxidase in this bacterium

J W De Gier; R J Van Spanning; L F Oltmann; A H Stouthamer

doi:10.1016/0014-5793(92)80829-6

Oxidation of methylamine by a Paracoccus denitrificans mutant impaired in the synthesis of the bc1 complex and the aa3-type oxidase. Evidence for the existence of an alternative cytochrome c oxidase in this bacterium

FEBS Lett. 1992 Jul 13;306(1):23-6. doi: 10.1016/0014-5793(92)80829-6.

Authors

J W De Gier¹, R J Van Spanning, L F Oltmann, A H Stouthamer

Affiliation

¹ Vrije Universiteit, Department of Microbiology, Amsterdam, The Netherlands.

PMID: 1321057
DOI: 10.1016/0014-5793(92)80829-6

Abstract

A Paracoccus denitrificans fbcC-ctaDII double mutant strain impaired in the synthesis of both the bc1 complex and the aa3-type oxidase has been constructed. This mutant strain, which is still able to grow on methylamine as sole carbon and energy source, exhibits unimpaired oxygen consumption with succinate, methylamine and endogenous substrates as electron donors. From kinetic studies of the oxidation and reduction rates of cytochromes c, it can be concluded that P. denitrificans contains a second cytochrome c oxidase, different from the aa3-type.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Electron Transport Complex III / biosynthesis*
Electron Transport Complex III / genetics
Electron Transport Complex IV / biosynthesis*
Electron Transport Complex IV / genetics
Electron Transport Complex IV / metabolism
Genes, Bacterial
Kinetics
Methylamines / metabolism*
Mutation*
Oxidation-Reduction
Oxygen Consumption
Paracoccus denitrificans / enzymology
Paracoccus denitrificans / genetics
Paracoccus denitrificans / metabolism*
Spectrophotometry, Ultraviolet
Substrate Specificity

Substances

Methylamines
methylamine
Electron Transport Complex IV
Electron Transport Complex III