Transcriptional regulation of the human insulin receptor promoter

J Biol Chem. 1992 Aug 25;267(24):17375-83.

Abstract

The ability of the human insulin receptor promoter to direct expression of a linked chloramphenicol acetyltransferase gene was assessed in transient transfections into HepG2 and Hela cells. A 5'-deletional analysis of the promoter showed that regions between -646 and -489 were important for the activity of the proximal promoter. In addition, a possible negative regulatory element was identified between -1311 and -877 and a positive element between -1823 and -1311. DNase I footprint and gel retardation analysis showed that multiple factors bind to the human insulin receptor promoter. In particular, DNase I protection patterns were observed over the Sp1 sites at -620 to -599 and -438 to -392, a TC box at -533, four homopyrimidine/homopurine sites clustered around -1150, and a site at -1420 that contains the motif TGGCCC which has been shown to bind the liver-specific transcription factor LF-A1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Binding Sites
  • Carcinoma, Hepatocellular
  • Cell Line
  • Chloramphenicol O-Acetyltransferase / biosynthesis
  • Chloramphenicol O-Acetyltransferase / genetics
  • Chromosome Deletion
  • Cloning, Molecular
  • DNA-Binding Proteins / metabolism
  • Deoxyribonuclease I
  • Gene Expression Regulation*
  • HeLa Cells
  • Hepatocyte Nuclear Factor 1
  • Hepatocyte Nuclear Factor 1-alpha
  • Hepatocyte Nuclear Factor 1-beta
  • Humans
  • Liver Neoplasms
  • Molecular Sequence Data
  • Nuclear Proteins*
  • Plasmids
  • Promoter Regions, Genetic*
  • Receptor, Insulin / biosynthesis
  • Receptor, Insulin / genetics*
  • Recombinant Fusion Proteins / biosynthesis
  • Restriction Mapping
  • Transcription Factors / metabolism*
  • Transcription, Genetic*
  • Transfection

Substances

  • DNA-Binding Proteins
  • HNF1A protein, human
  • HNF1B protein, human
  • Hepatocyte Nuclear Factor 1-alpha
  • Nuclear Proteins
  • Recombinant Fusion Proteins
  • Transcription Factors
  • Hepatocyte Nuclear Factor 1
  • Hepatocyte Nuclear Factor 1-beta
  • Chloramphenicol O-Acetyltransferase
  • Receptor, Insulin
  • Deoxyribonuclease I

Associated data

  • GENBANK/J05043
  • GENBANK/M84424
  • GENBANK/M92919
  • GENBANK/M92920
  • GENBANK/M93702
  • GENBANK/M93703
  • GENBANK/M93704
  • GENBANK/M93705
  • GENBANK/M94144
  • GENBANK/M94316