Detection and characterization of hepatitis C virus sequence in the serum of a patient with chronic HCV infection

Arch Virol Suppl. 1992:4:179-83. doi: 10.1007/978-3-7091-5633-9_37.

Abstract

A cDNA fragment corresponding to the nonstructural gene region of Hepatitis C virus was cloned and sequenced. cDNA was obtained by reverse transcription of viral RNA extracted from serum of a German patient with chronic post transfusion hepatitis. "Nested" PCR resulted in a cDNA fragment of 345 nt. The sequence showed a homology of 96% to the American prototype HCV.

Publication types

  • Comparative Study

MeSH terms

  • Base Sequence
  • Genes, Viral / genetics*
  • Hepacivirus / genetics*
  • Hepatitis C / epidemiology
  • Hepatitis C / genetics*
  • Hepatitis, Chronic / epidemiology
  • Hepatitis, Chronic / genetics*
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • RNA, Viral / blood*
  • Sequence Homology, Nucleic Acid

Substances

  • RNA, Viral