Abstract
A construct containing the 5' end of the human cytomegalovirus major immediate early gene fused to the human coagulation factor VIII cDNA was used to produce transgenic mice. Two out of five transgenic lines transcribed the construct. The expression was consistently seen in a limited number of tissues and was highest in muscle tissues. This is in contrast to the almost ubiquitous activity demonstrated in earlier studies with the IE-1 enhancer/promoter. Human factor VIII protein was detected immunochemically in muscle tissues at levels several times higher than in human plasma.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Northern
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Blotting, Southern
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Cloning, Molecular
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Cytomegalovirus / genetics*
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DNA / genetics
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DNA / isolation & purification
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Enhancer Elements, Genetic
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Factor VII / biosynthesis
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Factor VII / genetics*
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Female
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Gene Expression
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Genes, Viral*
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Humans
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Immunohistochemistry
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Inbred DBA
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Mice, Transgenic
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Muscles / cytology
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Muscles / physiology
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Organ Specificity
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Promoter Regions, Genetic
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RNA, Messenger / analysis
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RNA, Messenger / metabolism
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Recombinant Fusion Proteins / biosynthesis*
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Restriction Mapping
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Transcription, Genetic
Substances
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RNA, Messenger
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Recombinant Fusion Proteins
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Factor VII
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DNA