Abstract
Efficient expression systems are required for analysis of gene regulation and function in teleost fish. To develop such systems, a number of inducible or constitutive promoter and enhancer sequences of fish or higher vertebrate origin were tested for activity in a variety of fish cell lines and in embryos of the Japanese medaka fish (Oryzias latipes) and Xiphophorus. The activity of the different promoter-enhancer combinations were quantitated. Considerable differences were found for some constructs if tested in vitro or in vivo. Fro the data obtained, a set of expression vectors for basic research as well as for aquaculture purposes were established.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Genetically Modified / genetics*
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Blotting, Southern
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Carcinoma, Hepatocellular
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Chloramphenicol O-Acetyltransferase / biosynthesis
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Chloramphenicol O-Acetyltransferase / genetics
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DNA / chemistry
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Enhancer Elements, Genetic*
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Fishes / embryology
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Fishes / genetics*
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Gene Expression Regulation
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Genetic Vectors
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Humans
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Liver Neoplasms
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Metallothionein / genetics
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Microinjections
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Nucleic Acid Conformation
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Oryzias / embryology
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Oryzias / genetics
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Promoter Regions, Genetic*
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Thymidine Kinase / genetics
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Transfection / methods*
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Trout / genetics
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Tumor Cells, Cultured
Substances
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DNA
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Metallothionein
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Chloramphenicol O-Acetyltransferase
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Thymidine Kinase