Abstract
Freshly derived murine CD4+ T cells are divided into naive and memory cells based on the expression of CD45 isoforms. Cross-linking the T cell receptor CD3 complex either by plastic-bound anti-CD3 antibodies or the antibody presented on non-lymphoid Fc gamma receptor type II-positive Chinese hamster ovary cells in absence of competent antigen-presenting cells fails to activate naive cells to either secrete cytokines or to proliferate. In contrast, memory cells secrete their characteristic cytokines [interleukin (IL) 2, IL4, and interferon-gamma] and show significant proliferation to this stimulus. IL 1 however, is required for their optimal clonal expansion. Differential expression of IL 1 receptor mRNA in memory cells also correlate with their responsiveness to IL 1. Thus, these data reveal a basic difference in the requirements for activation of naive and memory CD4+ T cells.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Monoclonal / immunology
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Antigens, CD / analysis
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Antigens, Differentiation, T-Lymphocyte / analysis
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Base Sequence
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CD3 Complex
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CD4-Positive T-Lymphocytes / drug effects*
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CD4-Positive T-Lymphocytes / immunology
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Histocompatibility Antigens / analysis
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Immunologic Memory*
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Interleukin-1 / pharmacology*
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Leukocyte Common Antigens
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Mice
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Molecular Sequence Data
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RNA, Messenger / analysis
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Receptors, Antigen, T-Cell / analysis
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Receptors, Immunologic / genetics
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Receptors, Interleukin-1
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Receptors, Interleukin-2 / analysis
Substances
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Antibodies, Monoclonal
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Antigens, CD
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Antigens, Differentiation, T-Lymphocyte
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CD3 Complex
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Histocompatibility Antigens
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Interleukin-1
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RNA, Messenger
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Receptors, Antigen, T-Cell
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Receptors, Immunologic
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Receptors, Interleukin-1
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Receptors, Interleukin-2
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Leukocyte Common Antigens