Correlation between ELISA and recombinant immunoblot assay in serum samples positive for anti-HCV

J Formos Med Assoc. 1992 Jul;91(7):729-30.

Abstract

To determine the false-positivity of antibody to hepatitis C virus (HCV) (anti-C100-3), the correlation between ELISA optical density (OD), anti-HCV titer and recombinant immunoblot assay (RIBA) in 87 anti-HCV-positive sera was analyzed. The results showed that > 90% of the serum samples with an anti-HCV OD > 2.0 or with titers > 10 x were RIBA reactive. These findings are applicable in daily clinical practice.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Blood Donors
  • Child
  • Enzyme-Linked Immunosorbent Assay*
  • Female
  • Hepacivirus / immunology*
  • Hepatitis Antibodies / analysis*
  • Hepatitis C / diagnosis*
  • Hepatitis C / immunology
  • Hepatitis C Antibodies*
  • Hepatitis, Chronic / diagnosis
  • Hepatitis, Chronic / immunology
  • Humans
  • Immunoblotting*
  • Pregnancy
  • Reference Values

Substances

  • Hepatitis Antibodies
  • Hepatitis C Antibodies
  • anti-C100-3