Improved microscopic observation of mammalian cells on microcarriers by fluorescent staining

T J Nikolai; M V Peshwa; S Goetghebeur; W S Hu

doi:10.1007/BF00365430

Improved microscopic observation of mammalian cells on microcarriers by fluorescent staining

Cytotechnology. 1991 Feb;5(2):141-6. doi: 10.1007/BF00365430.

Authors

T J Nikolai¹, M V Peshwa, S Goetghebeur, W S Hu

Affiliation

¹ Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis 55455.

PMID: 1367155
DOI: 10.1007/BF00365430

Abstract

Many microcarriers used for the cultivation of animal cells do not allow for convenient microscopic observation of cell morphology and viability due to their optical properties. Using fluorescent viable stain combining fluorescein diacetate and ethidium bromide, we observed the distribution, morphology and viability of cells on various microcarriers.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Cell Division
Cell Line / cytology*
Cell Survival
Cricetinae
Ethidium*
Fluoresceins*
Fluorescent Dyes*
Microspheres
Vero Cells

Substances

Fluoresceins
Fluorescent Dyes
Ethidium
diacetylfluorescein