Modular integrated fluidized bed bioreactor technology

Biotechnology (N Y). 1991 Nov;9(11):1100-2. doi: 10.1038/nbt1191-1100.

Abstract

We describe the design and demonstrate the application of a modular integrated fluidized bed bioreactor system. Basically the system is a reactor vessel equipped with an extending cylinder and a liquid distributor plate. Instead of an external recirculation loop, as used in existing fluidized bed systems, a low shear stress impeller is used as the recirculation pump. The system has several unique features, such as modular exchangeable elements, efficient oxygenation and the option of operating as a stirred tank-, a packed bed- or a fluidized bed reactor. An example of a fluidized bed run using CHO-K1 cells is shown. Under standard culture conditions a 100-fold increase in cell density (up to 1.2 x 10(8) cells/ml) was achieved.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biotechnology / instrumentation*
  • Biotechnology / methods
  • CHO Cells
  • Cricetinae
  • Culture Techniques / instrumentation*
  • Culture Techniques / methods
  • HIV Antibodies / genetics*
  • HIV-1 / immunology
  • Humans
  • Recombination, Genetic

Substances

  • HIV Antibodies