Functional expression of GABAA chloride channels and benzodiazepine binding sites in baculovirus infected insect cells

D B Carter; D R Thomsen; W B Im; D J Lennon; D M Ngo; W Gale; H K Im; P H Seeburg; M W Smith

doi:10.1038/nbt0692-679

Functional expression of GABAA chloride channels and benzodiazepine binding sites in baculovirus infected insect cells

Biotechnology (N Y). 1992 Jun;10(6):679-81. doi: 10.1038/nbt0692-679.

Authors

D B Carter¹, D R Thomsen, W B Im, D J Lennon, D M Ngo, W Gale, H K Im, P H Seeburg, M W Smith

Affiliation

¹ Molecular Biology Research, Upjohn Company, Kalamazoo, MI 49007.

PMID: 1369398
DOI: 10.1038/nbt0692-679

Abstract

We have employed the baculovirus expression system for the production of insect cell membranes having GABA/benzodiazepine binding sites. Three recombinant baculoviruses each harboring a different GABAA receptor cDNA were introduced into insect cells by simultaneous infection. Infected cells expressed GABA responsive Cl- channels and benzodiazepine binding sites with the same pharmacological specificity as animal cells expressing these receptor subunits.

MeSH terms

Animals
Baculoviridae / genetics*
Benzodiazepinones / metabolism*
Binding Sites
Cell Line
Cell Membrane / metabolism*
Chloride Channels
DNA / genetics
Electrophysiology
Flumazenil / metabolism
Flunitrazepam / metabolism
Membrane Proteins / metabolism*
Moths / genetics
Moths / metabolism
Rats
Receptors, GABA-A / biosynthesis
Receptors, GABA-A / genetics*
Receptors, GABA-A / metabolism
Transfection*
gamma-Aminobutyric Acid / pharmacology

Substances

Benzodiazepinones
Chloride Channels
Membrane Proteins
Receptors, GABA-A
Flumazenil
gamma-Aminobutyric Acid
Flunitrazepam
DNA