Abstract
DNA amplification of specific sequences and subsequent oligonucleotide hybridization were used to search for Pneumocystis carinii in post-mortem lung samplings from non-immunosuppressed individuals ranging from 15 to 70 years of age. No P. carinii-specific DNA was detected in 45 DNA amplification reactions from 15 lungs.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Aged
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Electrophoresis, Agar Gel
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Ethidium
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Humans
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Lung / microbiology*
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Middle Aged
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Oligonucleotide Probes
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Pneumocystis / genetics
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Pneumocystis / isolation & purification*
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Polymerase Chain Reaction*
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Staining and Labeling
Substances
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Oligonucleotide Probes
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Ethidium