cDNA and gene sequences of wheat chloroplast sedoheptulose-1,7-bisphosphatase reveal homology with fructose-1,6-bisphosphatases

Eur J Biochem. 1992 May 1;205(3):1053-9. doi: 10.1111/j.1432-1033.1992.tb16873.x.

Abstract

The nucleotide sequence encoding the chloroplast enzyme, sedoheptulose-1,7-bisphosphatase [Sed(1,7)P2ase], was obtained from wheat cDNA and genomic clones. The transcribed region of the Sed(1,7)P2ase gene has eight exons (72-507 bp) and seven introns (85-626 bp) and encodes a precursor polypeptide of 393 amino acids. Comparison of the deduced amino acid sequence of Sed(1,7)P2ase with those of fructose-1,6-bisphosphatase [Fru(1,6)P2ase] enzymes from a variety of sources reveals 19% identity, rising to 42% if conservative changes are considered. Most importantly, the amino acid residues which form the active site of Fru(1,6)P2ase are highly conserved in the Sed(1,7)P2ase molecule, indicating a common catalytic mechanism. Interestingly, although the activities of both Sed(1,7)P2ase and chloroplast Fru(1,6)P2ase are modulated by light via the thioredoxin system, the amino acid sequence motif identified as having a role in this regulation in chloroplast Fru(1,6)P2ase is not found in the Sed(1,7)P2ase enzyme.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Binding Sites
  • Blotting, Northern
  • Chloroplasts / enzymology*
  • DNA / genetics*
  • Exons
  • Fructose-Bisphosphatase / genetics*
  • Fructose-Bisphosphatase / metabolism
  • Introns
  • Molecular Sequence Data
  • Phosphoric Monoester Hydrolases / genetics*
  • Phosphoric Monoester Hydrolases / metabolism
  • Poly A / genetics
  • RNA / genetics
  • RNA, Messenger
  • Restriction Mapping
  • Sequence Alignment
  • Sequence Homology, Nucleic Acid
  • Triticum / enzymology*

Substances

  • RNA, Messenger
  • Poly A
  • RNA
  • DNA
  • Fructose-Bisphosphatase
  • Phosphoric Monoester Hydrolases
  • sedoheptulose-bisphosphatase

Associated data

  • GENBANK/S87180
  • GENBANK/S87218
  • GENBANK/S87221
  • GENBANK/S87223
  • GENBANK/X62286
  • GENBANK/X65178
  • GENBANK/X65179
  • GENBANK/X65180
  • GENBANK/X65181
  • GENBANK/X65540