Abstract
We have isolated a cDNA clone by screening a rabbit kidney cortex cDNA library for expression of sodium-independent transport of L-arginine and L-alanine in Xenopus laevis oocytes. Expressed uptake relates to a single component of sodium-independent transport for dibasic and neutral amino acids. This transport activity resembles the functionally defined system b0,+ and carries cystine and dibasic amino acids with high affinity. The rBAT (b0,+ amino acid transporter-related) mRNA is found mainly in kidney and intestinal mucosa. It encodes a predicted 77.8-kDa protein with only one putative transmembrane domain and seven potential N-glycosylation sites. This protein could either be a constitutive element or a specific activator of system b0,+.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Amino Acids / metabolism*
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Amino Acids, Diamino / metabolism*
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Aminoisobutyric Acids / metabolism
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Animals
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Base Sequence
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Biological Transport
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Blotting, Northern
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Carrier Proteins / genetics*
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Carrier Proteins / metabolism
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Cloning, Molecular
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Cystine / metabolism*
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DNA / genetics*
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DNA / isolation & purification
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DNA / metabolism
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Kidney Cortex / metabolism
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Kidney Cortex / physiology*
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Membrane Proteins / genetics*
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Membrane Proteins / metabolism
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Molecular Sequence Data
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Oocytes / metabolism
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Oocytes / physiology
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RNA / genetics
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RNA / isolation & purification
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Rabbits
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Rats
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Xenopus laevis
Substances
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Amino Acids
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Amino Acids, Diamino
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Aminoisobutyric Acids
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Carrier Proteins
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Membrane Proteins
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2-aminoisobutyric acid
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Cystine
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RNA
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DNA