Abstract
The population architecture of sulfidogenic biofilms established in anaerobic fixed-bed bioreactors was characterized by selective polymerase chain reaction amplification and fluorescence microscopy. A region of the 16S rRNA common to resident sulfate-reducing bacteria was selectively amplified by the polymerase chain reaction. Sequences of amplification products, with reference to a collection of 16S rRNA sequences representing most characterized sulfate-reducing bacteria, were used to design both general and specific hybridization probes. Fluorescent versions of these probes were used in combination with fluorescence microscopy to visualize specific sulfate-reducing bacterial populations within developing and established biofilms.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Base Sequence
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DNA Probes
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Microscopy, Fluorescence
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Molecular Sequence Data
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Nucleic Acid Hybridization
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Oxidation-Reduction
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Phylogeny
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Polymerase Chain Reaction
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RNA, Bacterial
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RNA, Ribosomal, 16S / analysis
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RNA, Ribosomal, 16S / genetics*
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Sulfates / metabolism*
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Sulfur-Reducing Bacteria / classification
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Sulfur-Reducing Bacteria / isolation & purification*
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Sulfur-Reducing Bacteria / metabolism
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Temperature
Substances
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DNA Probes
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RNA, Bacterial
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RNA, Ribosomal, 16S
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Sulfates
Associated data
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GENBANK/J01695
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GENBANK/M11223
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GENBANK/M21413
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GENBANK/M34113
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GENBANK/M34114
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GENBANK/M34117
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GENBANK/M34399
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GENBANK/M34400
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GENBANK/M34401
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GENBANK/M34402
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GENBANK/M34403
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GENBANK/M34404
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GENBANK/M34405
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GENBANK/M34407
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GENBANK/M34408
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GENBANK/M34412
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GENBANK/M34413
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GENBANK/M36507
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GENBANK/M37315
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GENBANK/M37316
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GENBANK/M80617
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GENBANK/M80618
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GENBANK/N34118
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GENBANK/X00007
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GENBANK/X00134
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GENBANK/X03235
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GENBANK/X03772
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GENBANK/X05567
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GENBANK/Y00275