A simple procedure for recovering the denaturing effect of methylmercury in agarose gel electrophoresis

P Fernández-Silva; J A Enriquez; J Montoya

A simple procedure for recovering the denaturing effect of methylmercury in agarose gel electrophoresis

Biotechniques. 1992 Apr;12(4):480-2.

Authors

P Fernández-Silva¹, J A Enriquez, J Montoya

Affiliation

¹ Departamento de Bioquímica y Biología Molecular y Celular, Facultad de Veterinaria, Zaragoza, Spain.

PMID: 1380250

Abstract

A simple and rapid procedure for recovering the denaturing effect of methylmercuric hydroxide in agarose gel electrophoresis is described. The procedure consisted of the treatment of the commercial methylmercuric hydroxide solutions with Amberlite, a mixture of anion- and cation-exchange resins. This treatment greatly improved the resolution of RNA species when fractionated by electrophoresis through agarose-CH3HgOH slab gel.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Electrophoresis, Agar Gel / methods*
Methylmercury Compounds / adverse effects*
Nucleic Acid Denaturation / drug effects*
RNA
Rats
Resins, Synthetic

Substances

Methylmercury Compounds
Resins, Synthetic
RNA
amberlite
methylmercury hydroxide