Isolation of mouse Lyt2- mutant cells using magnetic particles

J Immunol Methods. 1992 Oct 2;154(2):211-6. doi: 10.1016/0022-1759(92)90194-x.

Abstract

A procedure is described for the selection of mouse Lyt2- mutant cells. The procedure is based upon repeated cycles of selection with rat monoclonal antibodies to the Lyt2 antigen and magnetic particles coated with goat anti-mouse IgG. Stable Lyt2- mutant clones were derived from cells previously mutagenized with X rays and, at a lower frequency, also from non-mutagenized cells. The procedure can be used to rescue Lyt2- cells when the ratio of Lyt2+:Lyt2- cells is 10(4):1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Ly / analysis
  • Antigens, Ly / genetics*
  • Cell Separation / methods*
  • Immunologic Techniques
  • In Vitro Techniques
  • Lymphocyte Depletion
  • Magnetics
  • Mice
  • Mutation
  • T-Lymphocyte Subsets / cytology*

Substances

  • Antigens, Ly