Abstract
We investigated the influence of CKS-17, a synthetic heptadecapeptide that corresponds to a highly conserved domain of the immunosuppressive retroviral envelope protein p15E, on staphylococcal enterotoxin B (SEB)-induced TNF-alpha gene expression in human peripheral blood mononuclear cells and highly purified human monocyte preparations, as well as the production of TNF-alpha protein, using human peripheral blood mononuclear cells. RNA hybridization studies show that CKS-17 inhibits SEB-induced TNF-alpha mRNA accumulation in human peripheral blood mononuclear cells and human monocytes. CKS-17 is also shown to be highly suppressive for SEB-induced production of TNF-alpha proteins. Similarly, CKS-17 inhibits expression of SEB-induced IFN-gamma mRNA in human peripheral blood mononuclear cells. These results suggest that CKS-17 down-regulates both TNF-alpha and IFN-gamma production at mRNA level.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Blotting, Northern
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Down-Regulation / drug effects*
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Enterotoxins / pharmacology
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Gene Expression / drug effects
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Humans
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Immunoblotting
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Intercellular Signaling Peptides and Proteins
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Interferon-gamma / biosynthesis
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Interferon-gamma / genetics*
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Molecular Sequence Data
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Monocytes / drug effects
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Monocytes / metabolism
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Monocytes / physiology
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Peptides / pharmacology*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism*
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Retroviridae Proteins, Oncogenic / pharmacology*
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Tumor Necrosis Factor-alpha / biosynthesis
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Tumor Necrosis Factor-alpha / genetics*
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Viral Envelope Proteins / pharmacology*
Substances
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Enterotoxins
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Intercellular Signaling Peptides and Proteins
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Peptides
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RNA, Messenger
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Retroviridae Proteins, Oncogenic
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Tumor Necrosis Factor-alpha
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Viral Envelope Proteins
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enterotoxin B, staphylococcal
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Interferon-gamma
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CKS 17