Abstract
Synovial fluid (SF) mononuclear cells from a patient with Chlamydia trachomatis induced acute Reiter's disease were directly by limiting dilution in a representative protocol using phytohemagglutinin in the cloning medium. Out of 76 alpha beta-TCR+ CD4+ T lymphocyte clones, 7 were shown to specifically recognize C. trachomatis in a proliferation assay. The antigen recognition of these clones was HLA-DP restricted. Unexpectedly, 2 HLA-DR restricted clones showed a proliferative response to Yersinia enterocolitica O3, though the patient had no history of yersinia infection. The high frequency of SF derived T cells with specificity for species-specific chlamydial antigens and the limited diversity of HLA class II restriction of these clones may indicate an oligoclonal synovial T cell response to persistent intraarticular chlamydia.
Publication types
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Case Reports
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Antigens, Bacterial / analysis
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Arthritis, Infectious / immunology
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Arthritis, Infectious / pathology
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Arthritis, Reactive / etiology
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Arthritis, Reactive / immunology*
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Arthritis, Reactive / pathology
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CD4 Antigens / analysis
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CD4 Antigens / immunology
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Cartilage, Articular / immunology
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Cartilage, Articular / pathology
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Chlamydia Infections / immunology
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Chlamydia trachomatis / immunology*
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Chlamydia trachomatis / physiology*
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Clone Cells
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HLA-DP Antigens / immunology*
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HLA-DR Antigens / immunology
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Humans
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Male
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Phytohemagglutinins
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Receptors, Antigen, T-Cell, alpha-beta / analysis
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Receptors, Antigen, T-Cell, alpha-beta / immunology
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Species Specificity
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Synovial Fluid / cytology*
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T-Lymphocytes / immunology*
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T-Lymphocytes / pathology
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Yersinia Infections / immunology
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Yersinia Infections / pathology
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Yersinia enterocolitica / immunology
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Yersinia enterocolitica / physiology
Substances
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Antigens, Bacterial
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CD4 Antigens
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HLA-DP Antigens
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HLA-DR Antigens
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Phytohemagglutinins
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Receptors, Antigen, T-Cell, alpha-beta