Abstract
Two new protocols for the three-dimensional, triple resonance, constant-time HCA(CO)N NMR experiment are presented that significantly increase the experimental resolution attainable in the C alpha frequency dimension. Experimental verification of the new experiments is provided by spectra of the IIA domain of glucose permease from Bacillus subtilis.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Bacillus subtilis / enzymology
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Magnetic Resonance Spectroscopy / methods*
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Mathematics
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Phosphoenolpyruvate Sugar Phosphotransferase System / chemistry*
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Protein Conformation*
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Proteins / chemistry*
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Time Factors
Substances
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Proteins
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Phosphoenolpyruvate Sugar Phosphotransferase System
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phosphoenolpyruvate-glucose phosphotransferase