Agglomeration of calcium oxalate monohydrate in synthetic urine

Br J Urol. 1992 Sep;70(3):240-6. doi: 10.1111/j.1464-410x.1992.tb15724.x.

Abstract

The development of agglomerated particles of calcium oxalate monohydrate (COM) on the semi-batch precipitation from a synthetic urine carried out at physiological conditions (37 degrees C, pH = 5.5) was studied by optical and electron scanning microscopy. COM agglomerates develop by primary and secondary agglomeration proceeding simultaneously; the latter mechanism is, however, less important than the former. Citrate ions modify slightly the COM crystal shape and inhibit primary agglomeration. Mucin particles serve as a substrate for preferential formation (nucleation) of new COM crystals. The structure of formed agglomerates closely resembles that of a certain type of COM renal calculi. A combination of primary agglomeration of crystals forming stones and nucleation of new crystals on a mucoprotein layer partially covering their surface constitutes the possible mechanism of such stone development. Experimental data support this mechanism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium Oxalate / urine*
  • Crystallization
  • Microscopy, Electron, Scanning
  • Urinary Calculi / chemistry
  • Urine

Substances

  • Calcium Oxalate