Chromosome painting using chromosome-specific probes from flow-sorted pig chromosomes

C F Langford; H Telenius; N P Carter; N G Miller; E M Tucker

doi:10.1159/000133411

Chromosome painting using chromosome-specific probes from flow-sorted pig chromosomes

Cytogenet Cell Genet. 1992;61(3):221-3. doi: 10.1159/000133411.

Authors

C F Langford¹, H Telenius, N P Carter, N G Miller, E M Tucker

Affiliation

¹ AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, UK.

PMID: 1424813
DOI: 10.1159/000133411

Abstract

Biotinylated chromosome-specific probes were prepared from flow-sorted pig chromosomes 1, 13, 18, X, and Y using the degenerate oligonucleotide-primed polymerase chain reaction. Probes prepared in this way can be used to confirm the identity of chromosomes in the bivariate pig flow karyotype and in pig x mouse somatic cell hybrids.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Flow Cytometry*
Hybrid Cells
In Situ Hybridization*
Karyotyping / methods*
Molecular Sequence Data
Oligonucleotide Probes / genetics*
Polymerase Chain Reaction
Swine

Substances

Oligonucleotide Probes