Abstract
Using single-strand conformation polymorphism electrophoresis, heteroduplex analysis, and direct sequencing, we have searched for possible disease-causing mutations in the adRP family in which we originally found tight linkage of the disease to 6p. We have now identified a single base change in exon 2, which results in the replacement of a serine residue at codon 212 for a glycine residue. The mutation cosegregates with the disease with a lod score of 12.1 at theta = 0.0.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Chromosomes, Human, Pair 6*
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DNA, Single-Stranded
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Genes, Dominant*
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Genetic Linkage
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Humans
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Intermediate Filament Proteins / genetics*
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Membrane Glycoproteins*
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Molecular Sequence Data
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Mutation*
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Nerve Tissue Proteins*
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Pedigree
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Peripherins
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Retinitis Pigmentosa / genetics*
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Rhodopsin / genetics*
Substances
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DNA, Single-Stranded
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Intermediate Filament Proteins
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Membrane Glycoproteins
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Nerve Tissue Proteins
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PRPH protein, human
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PRPH2 protein, human
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Peripherins
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Rhodopsin