Benzoangelicins: new monofunctional DNA photobinding agents

J Photochem Photobiol B. 1992 Jun 30;14(1-2):81-93. doi: 10.1016/1011-1344(92)85084-8.

Abstract

4,6-Dimethylbenzoangelicin, obtained by fusing a benzene ring at the furan side of 4,6-dimethylangelicin, was studied in terms of crystal structure and interactions with DNA in both ground and excited states. 4,6-Dimethylbenzoangelicin has a planar structure and forms a molecular complex with DNA, undergoing intercalation inside the double helix. Under UVA irradiation, it photoconjugates covalently with the macromolecule, showing a DNA photobinding rate slightly lower than that of 8-methoxypsoralen, involving however only its 3,4 double bond, i.e. behaving as a pure monofunctional agent. The parameters of dark binding and photobinding were determined, and two C4 cycloadducts with thymine were isolated and characterized.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cross-Linking Reagents / chemistry*
  • DNA / chemistry*
  • DNA / isolation & purification
  • DNA / metabolism
  • Furocoumarins / chemistry*
  • Furocoumarins / metabolism
  • Kinetics
  • Models, Molecular
  • Molecular Conformation
  • Nucleic Acid Conformation
  • Radiation-Sensitizing Agents / chemistry*
  • Spectrophotometry, Ultraviolet
  • X-Ray Diffraction

Substances

  • Cross-Linking Reagents
  • Furocoumarins
  • Radiation-Sensitizing Agents
  • 4,6-dimethylbenzoangelicin
  • DNA