Abstract
The influences of protein kinase inhibitors, K-252a and staurosporine, on the neurotrophic effects of epidermal growth factor (EGF) were investigated in dissociated cell cultures of the hippocampus and cerebellum of fetal rats. Addition of 1 ng/ml EGF enhanced the survival of cultured neurons of both brain regions. Both K-252a (10-200 nM) and staurosporine (1-100 nM) blocked the survival-promoting effects of EGF in a concentration-dependent manner. These results suggest that activation of protein kinase(s) is involved in the neurotrophic effects of EGF.
MeSH terms
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Alkaloids / pharmacology
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Animals
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Brain / cytology
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Brain / drug effects*
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Carbazoles / pharmacology
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Cell Survival / drug effects
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Cells, Cultured
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Epidermal Growth Factor / antagonists & inhibitors
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Epidermal Growth Factor / pharmacology*
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Indole Alkaloids
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Neurons / cytology
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Neurons / drug effects
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Protein Kinase C / antagonists & inhibitors*
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Rats
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Recombinant Proteins / antagonists & inhibitors
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Recombinant Proteins / pharmacology
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Staurosporine
Substances
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Alkaloids
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Carbazoles
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Indole Alkaloids
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Recombinant Proteins
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Epidermal Growth Factor
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staurosporine aglycone
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Protein Kinase C
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Staurosporine