Abstract
The topology of the disulfides in native heparin-binding neurotrophic factor (HBNF) isolated from bovine brain was studied by proteolytic digestion using trypsin, Asp-N endoproteinase and chymotrypsin and peptide mapping. Disulfide-linked peptides were identified by automated Edman degradation. It has been shown that there are disulfide bonds between Cys15-Cys44, Cys23-Cys53, Cys30-Cys57, Cys67-Cys99 and Cys77-Cys109.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Brain Chemistry
-
Carrier Proteins / chemistry*
-
Carrier Proteins / genetics
-
Carrier Proteins / isolation & purification
-
Cattle
-
Chymotrypsin
-
Cysteine Endopeptidases*
-
Cytokines / chemistry*
-
Cytokines / genetics
-
Cytokines / isolation & purification
-
Disulfides / chemistry
-
Endopeptidases
-
Molecular Sequence Data
-
Molecular Structure
-
Molecular Weight
-
Peptide Mapping
-
Protein Structure, Secondary
-
Trypsin
Substances
-
Carrier Proteins
-
Cytokines
-
Disulfides
-
pleiotrophin
-
Endopeptidases
-
Chymotrypsin
-
Trypsin
-
Cysteine Endopeptidases
-
asparaginylendopeptidase