Immunomodulation of streptococcal cell wall-induced arthritis. Identification of inflammatory cells and regulatory T cell subsets by mercuric chloride and in vivo CD8 depletion

Eur J Immunol. 1992 Dec;22(12):3091-5. doi: 10.1002/eji.1830221210.

Abstract

Streptococcal cell wall (SCW)-induced arthritis is a chronic, erosive polyarthritis which can be induced in susceptible Lewis rats by one intraperitoneal injection of a sterile, aqueous suspension of SCW. The chronic phase of the disease is dependent on T cells. Mercuric chloride is an immunomodulating agent, causing autoimmunity in BN rats, but an OX8+ cell-mediated immunosuppression in Lewis rats. Therefore, we investigated the effect of mercuric chloride, whether or not combined with in vivo OX8 depletion, on SCW-induced arthritis in Lewis rats. We show that (a) depletion of OX8+ cells leads to a more chronic arthritis with a more rapid onset, (b) treatment with mercuric chloride induces a rapidly developing disease which is not chronic, and (c) treatment with mercuric chloride and OX8+ cell depletion induces an arthritis with a very rapid onset and enhanced chronicity. Together with histological data this suggests an important role for OX8+ T cells in controlling both the acute and chronic phase of the disease. In addition, mercuric chloride seems to induce an early activation of T cells resulting in an enhanced onset of disease, which is controlled later by enhanced activation of OX8+ cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adjuvants, Immunologic / pharmacology*
  • Animals
  • Antibodies, Monoclonal / immunology*
  • Arthritis, Infectious / immunology*
  • Arthritis, Infectious / pathology
  • CD8 Antigens / physiology*
  • Cell Wall / immunology
  • Female
  • Lymphocyte Activation / drug effects*
  • Mercuric Chloride / pharmacology*
  • Rats
  • Rats, Inbred Lew
  • Streptococcal Infections / immunology*
  • Streptococcal Infections / pathology
  • T-Lymphocyte Subsets / physiology*

Substances

  • Adjuvants, Immunologic
  • Antibodies, Monoclonal
  • CD8 Antigens
  • Mercuric Chloride