Abstract
The potential of the major structural protein DraE of Escherichia coli Dr fimbriae has been used to display an 11-amino-acid peptide of glycoprotein D derived from herpes simplex virus (HSV) type 1. The heterologous sequence mimicking an epitope from glycoprotein D was inserted in one copy into the draE gene in place of a predicted 11-amino-acid sequence in the N-terminal region of surface-exposed domain 2 within the conserved disulfide loop (from Cys21 to Cys53). The inserted epitope was displayed on the surface of the chimeric DraE protein as evidenced by immunofluorescence and was recognized by monoclonal antibodies to the target HSV glycoprotein D antigen. Conversely, immunization of rabbits with purified chimeric Dr-HSV fimbriae resulted in a serum that specifically recognized the 11-amino-acid epitope of HSV glycoprotein D, indicating the utility of the strategy employed.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adhesins, Bacterial / genetics
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Adhesins, Bacterial / immunology
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Amino Acid Sequence
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Animals
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Antibodies, Monoclonal
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Antigens, Viral / genetics
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Base Sequence
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DNA, Recombinant / genetics
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Epitopes / genetics
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Escherichia coli / genetics
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Escherichia coli / immunology
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / immunology
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Fimbriae, Bacterial / genetics
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Fimbriae, Bacterial / immunology
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Herpesvirus 1, Human / genetics*
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Herpesvirus 1, Human / immunology*
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Herpesvirus Vaccines / genetics
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Herpesvirus Vaccines / immunology
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Humans
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In Vitro Techniques
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Models, Immunological
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Peptide Library
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Rabbits
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / immunology
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Vaccines, Synthetic / genetics*
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Vaccines, Synthetic / immunology*
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Viral Envelope Proteins / genetics*
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Viral Envelope Proteins / immunology*
Substances
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Adhesins, Bacterial
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Antibodies, Monoclonal
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Antigens, Viral
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DNA, Recombinant
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DraE protein, E coli
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Epitopes
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Escherichia coli Proteins
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Herpesvirus Vaccines
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Peptide Library
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Recombinant Fusion Proteins
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Vaccines, Synthetic
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Viral Envelope Proteins
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glycoprotein D, Human herpesvirus 1