Parenchymal organ, and not splenic, immunity correlates with host survival during disseminated candidiasis

Infect Immun. 2003 Oct;71(10):5756-64. doi: 10.1128/IAI.71.10.5756-5764.2003.

Abstract

We examined the relationship between host survival and renal and splenic immune responses in a murine model of hematogenously disseminated candidiasis. Male BALB/c mice were infected via tail vein injection with wild-type C. albicans or with an isogenic, Deltaefg1/Deltaefg1 hypha-deficient mutant. Host survival, organ fungal burden, intracellular cytokine content of splenic and kidney lymphocytes, and whole-organ cytokine profiles were determined. Wild-type C. albicans induced type 2 splenocyte responses with both nonfatal and fatal inocula. In the kidney, conversely, wild-type inocula causing no or low mortality induced type 1 responses and 100% fatal inocula induced type 2 or interleukin-10 (IL-10)-dominant responses. Hypha-deficient mutant C. albicans caused no or low mortality while inducing type 1 responses in both the spleen and kidney. To our knowledge, this is the first demonstration that host survival during systemic infection correlates with the type of immune response engendered in a nonlymphoid, parenchymal organ and not with the response in the spleen. Furthermore, the results provide in vivo confirmation that hyphal formation by C. albicans induces type 2 or IL-10-dominant host responses in tissues.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Candida albicans / genetics
  • Candida albicans / growth & development
  • Candida albicans / immunology
  • Candida albicans / pathogenicity
  • Candidiasis / immunology*
  • Candidiasis / microbiology
  • Colony Count, Microbial
  • Interferon-gamma / metabolism
  • Interleukin-10 / metabolism
  • Interleukin-4 / metabolism
  • Kidney / immunology
  • Kidney / microbiology
  • Lymphocyte Activation
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mutation
  • Organ Specificity
  • Prognosis
  • Spleen / immunology
  • Spleen / microbiology
  • Th1 Cells / immunology
  • Th2 Cells / immunology
  • Virulence

Substances

  • Interleukin-10
  • Interleukin-4
  • Interferon-gamma