Advanced hemostatic dressing development program: animal model selection criteria and results of a study of nine hemostatic dressings in a model of severe large venous hemorrhage and hepatic injury in Swine

J Trauma. 2003 Sep;55(3):518-26. doi: 10.1097/01.TA.0000075336.92129.27.

Abstract

Background: An advanced hemostatic dressing is needed to augment current methods for the control of life-threatening hemorrhage. A systematic approach to the study of dressings is described. We studied the effects of nine hemostatic dressings on blood loss using a model of severe venous hemorrhage and hepatic injury in swine.

Methods: Swine were treated using one of nine hemostatic dressings. Dressings used the following primary active ingredients: microfibrillar collagen, oxidized cellulose, thrombin, fibrinogen, propyl gallate, aluminum sulfate, and fully acetylated poly-N-acetyl glucosamine. Standardized liver injuries were induced, dressings were applied, and resuscitation was initiated. Blood loss, hemostasis, and 60-minute survival were quantified.

Results: The American Red Cross hemostatic dressing (fibrinogen and thrombin) reduced (p < 0.01) posttreatment blood loss (366 mL; 95% confidence interval, 175-762 mL) and increased (p < 0.05) the percentage of animals in which hemostasis was attained (73%), compared with gauze controls (2,973 mL; 95% confidence interval, 1,414-6,102 mL and 0%, respectively). No other dressing was effective. The number of vessels lacerated was positively related to pretreatment blood loss and negatively related to hemostasis.

Conclusion: The hemorrhage model allowed differentiation among topical hemostatic agents for severe hemorrhage. The American Red Cross hemostatic dressing was effective and warrants further development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bandages*
  • Blood Pressure
  • Collagen / therapeutic use
  • Confidence Intervals
  • Disease Models, Animal*
  • Female
  • Fibrinogen / therapeutic use
  • Hemorrhage / therapy*
  • Hemostatic Techniques*
  • Hemostatics / therapeutic use*
  • Liver / injuries*
  • Male
  • Propyl Gallate / therapeutic use
  • Swine
  • Thrombin / therapeutic use

Substances

  • Hemostatics
  • Propyl Gallate
  • Fibrinogen
  • Collagen
  • Thrombin