Abstract
HIV-1 and other retroviruses exit infected cells by budding from the plasma membrane, a process requiring membrane fission. The primary late assembly (L) domain in the p6 region of HIV-1 Gag mediates the detachment of the virion by recruiting host Tsg101, a component of the class E vacuolar protein sorting (Vps) machinery. We now show that HIV Gag p6 contains a second region involved in L domain function that binds AIP1, a homolog of the yeast class E Vps protein Bro1. Further, AIP1 interacts with Tsg101 and homologs of a subunit of the yeast class E Vps protein complex ESCRT-III. AIP1 also binds to the L domain in EIAV p9, and this binding correlates perfectly with L domain function. These observations identify AIP1 as a component of the viral budding machinery, which serves to link a distinct region in the L domain of HIV-1 p6 and EIAV p9 to ESCRT-III.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adenosine Triphosphatases / genetics
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Adenosine Triphosphatases / metabolism
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Binding Sites / genetics
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Cell Membrane / metabolism
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Cell Membrane / virology
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Endosomal Sorting Complexes Required for Transport
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Gene Products, gag / genetics
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Gene Products, gag / metabolism*
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HIV-1 / genetics
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HIV-1 / metabolism*
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HeLa Cells
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Humans
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Infectious Anemia Virus, Equine / genetics
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Infectious Anemia Virus, Equine / metabolism*
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Microfilament Proteins / genetics
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Microfilament Proteins / metabolism*
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Microscopy, Electron
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism
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Protein Binding / genetics
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Protein Structure, Tertiary / genetics
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism
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Simian Immunodeficiency Virus / genetics
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Simian Immunodeficiency Virus / metabolism
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Vesicular Transport Proteins
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Virus Shedding / physiology*
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gag Gene Products, Human Immunodeficiency Virus
Substances
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CHMP1A protein, human
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DNA-Binding Proteins
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Endosomal Sorting Complexes Required for Transport
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Gene Products, gag
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Microfilament Proteins
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Nuclear Proteins
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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Tsg101 protein
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VPS4 protein, S cerevisiae
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Vesicular Transport Proteins
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actin interacting protein 1
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gag Gene Products, Human Immunodeficiency Virus
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p6 gag protein, Human immunodeficiency virus 1
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Adenosine Triphosphatases