Lack of efficacy of 'naked' small interfering RNA applied directly to rat brain

Acta Physiol Scand. 2003 Oct;179(2):173-7. doi: 10.1046/j.1365-201X.2003.01188.x.

Abstract

The intrastriatal infusions of 'naked' small interfering RNA (siRNA) targeted to dopamine D1 receptors (1.0-10.0 nmol over 3 days) did not reduce dopamine D1 receptor messenger RNA levels or receptor protein, assessed by [125I] SCH 23982 binding in intact rats. This was in contrast to results in vitro where a 76% reduction in dopamine D1 receptor ligand binding could be observed.

Conclusion: The results suggest that synthetic siRNA, when applied directly to rat brain, is not capable of inducing RNA interference.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autoradiography / methods
  • Brain / metabolism
  • Brain / physiology
  • In Situ Hybridization / methods
  • Male
  • Oligonucleotide Probes
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • RNA, Small Interfering / genetics*
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Dopamine D1 / antagonists & inhibitors
  • Receptors, Dopamine D1 / genetics*
  • Transfection

Substances

  • Oligonucleotide Probes
  • RNA, Messenger
  • RNA, Small Interfering
  • Receptors, Dopamine D1