Abstract
The alternative oxidase (AOX) is a terminal oxidase present in the respiratory chain of all plants as well as some yeasts and trypanosomes, but has not previously been found in a prokaryote. We have identified an AOX homologue in Novosphingobium aromaticivorans, the first AOX found in a prokaryote. We have cloned the gene for the N. aromaticivorans AOX and showed it to have a terminal oxidase activity when heterologously expressed in Escherichia coli. We have also shown that this novel AOX is expressed in N. aromaticivorans cells, and that its expression level is greatly influenced by the oxygen level and carbon source of the growth media.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alphaproteobacteria / enzymology*
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Alphaproteobacteria / genetics
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Alphaproteobacteria / growth & development
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Cloning, Molecular
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Culture Media
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Escherichia coli / genetics
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Gene Expression Regulation, Bacterial
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Gene Expression Regulation, Enzymologic
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Genes, Bacterial
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Genetic Complementation Test
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Mitochondrial Proteins
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Oxidoreductases / genetics
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Oxidoreductases / metabolism*
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Plant Proteins
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
Substances
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Culture Media
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Mitochondrial Proteins
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Plant Proteins
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Recombinant Fusion Proteins
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Oxidoreductases
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alternative oxidase