Abstract
The speed of synaptic vesicle recycling determines the efficacy of neurotransmission during repetitive stimulation. Synaptotagmins are synaptic C(2)-domain proteins that are involved in exocytosis, but have also been linked to endocytosis. We now demonstrate that upon expression in transfected neurons, a short splice variant of synaptotagmin 7 that lacks C(2)-domains accelerates endocytic recycling of synaptic vesicles, whereas a longer splice variant that contains C(2)-domains decelerates recycling. These results suggest that alternative splicing of synaptotagmin 7 acts as a molecular switch, which targets vesicles to fast and slow recycling pathways.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing*
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Animals
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Animals, Newborn
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Calcium-Binding Proteins*
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Cells, Cultured
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Genetic Variation*
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Hippocampus
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Membrane Glycoproteins / genetics*
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Membrane Glycoproteins / physiology
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Membrane Potentials / physiology
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Microscopy, Electron
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Nerve Tissue Proteins / genetics*
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Nerve Tissue Proteins / physiology
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Neurons / cytology
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Neurons / physiology*
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Rats
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Rats, Sprague-Dawley
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Receptors, Cell Surface / genetics
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Recombinant Proteins / metabolism
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Synapses / ultrastructure
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Synaptic Vesicles / physiology*
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Synaptic Vesicles / ultrastructure*
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Synaptotagmins
Substances
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Calcium-Binding Proteins
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Membrane Glycoproteins
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Nerve Tissue Proteins
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Receptors, Cell Surface
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Recombinant Proteins
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Syt7 protein, rat
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Synaptotagmins