A novel multiplex PCR system consisting of Y-STRs DYS441, DYS442, DYS443, DYS444, and DYS445

J Forensic Sci. 2003 Sep;48(5):1088-90.

Abstract

We have developed a new sensitive multiplex PCR system consisting of five male-specific and polymorphic tetranucleotide STRs--DYS441 (GDB: 10013873), DYS442 (GDB: 10030304), DYS443 (GDB: 10807127), DYS444 (GDB: 10807128), and DYS445 (GDB: 10807129) on the Y chromosome. Fifty pg DNA per 10 microL reaction volume was required for the correct typing of five STRs. Using this system, the five Y-STRs were correctly typed from blood and semen stains that had been stored for several years at room temperature.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Stains
  • Chromosomes, Human, Y*
  • DNA / analysis*
  • DNA Fingerprinting / methods*
  • DNA Primers
  • Female
  • Forensic Medicine / methods
  • Humans
  • Male
  • Polymerase Chain Reaction / methods*
  • Polymorphism, Genetic
  • Semen / chemistry
  • Tandem Repeat Sequences*

Substances

  • DNA Primers
  • DNA